Skip to main content

Table 1 Summary of SIRT3 steady-state kinetic analyses

From: Role of SIRT3 in the regulation of redox balance during oral carcinogenesis

 

SIRT3

 

WT

V208I

P345R

H248Y

NAD + V max (pmole/min/mg)

16.03 ± 0.02

12.14 ± 0.06

15.94 ± 0.14

1.08 ± 0.16

     K M (mM)

0.88 ± 0.01

1.07 ± 0.03

0.88 ± 0.07

N.D.

Catalytic efficiency (V max /K M )

18.21

11.35

18.11

N.D.

Peptide V max (pmole/min/mg)

17.5 ± 0.69

12.5 ± 0.03

17.32 ± 0.45

1.32 ± 0.11

     K M (mM)

147.3 ± 12.4

153.4 ± 17.5

146.8 ± 10.8

54.2 ± 14.0

Catalytic efficiency (V max /K M )

0.11

0.07

0.12

0.02

  1. Rates of activity were measured as a function of NAD+ concentration or peptide substrate concentration, as measured by fluorescence intensity; n = 3 independent experiments (mean ± SD).
Back to article page

Molecular Cancer

ISSN: 1476-4598