Hormone activates the VEGF minimal core promoter region lacking ARE sites. (A) Schematic diagram of an 88bp region of the VEGF core promoter showing four predicted Sp1 (black box) and Egr1 (white box) binding sites. (B) 22Rv1 cells were transfected with V88 followed by treatment with 0nM or 5nM R1881 as described above. Luciferase assays were performed as previously described. (C) 22Rv1 cells were transfected as in (B) except pre-treated with 0μM or 10μM casodex for 2 hours. Luciferase activity is shown relative to average normalized luciferase activity in the absence of hormone. Experiments were repeated three times in triplicate. Significance was determined by Student’s t-test (*p<0.05, ** p<0.01).