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Figure 3 | Molecular Cancer

Figure 3

From: Orthotopic transplantation of retinoblastoma cells into vitreous cavity of zebrafish for screening of anticancer drugs

Figure 3

Transplanted retinoblastoma cells are successfully isolated and maintain their characteristics as tumor cells. Transplantation and further isolation of retinoblastoma cells do not induce morphological changes in retinoblastoma cells Before injection, (A; After transplantation and isolation, B). Scale bar, 100 μm. Western blotting for glial differentiation marker, GFAP and neuronal differentiation marker, NSE demonstrates no definite change between preinjected and isolated retinoblastoma cells (C). RT-PCR shows stable expression of CRALBP gene, a marker for retinal constituent cells (D). As with the first injection of retinoblastoma cells, reinjection of isolated cells demonstrates the stable tumor population 2 days after the injection First injection, (E; Reinjection of isolated cells, F). Scale bar, 100 μm. Rb1-Zf, isolated cells from zebrafish into which SNUOT-Rb1 cells were injected.

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