Figure 4

A novel orthotopic retinoblastoma model in zebrafish can be utilized for screening of anticancer drugs. Retinoblastoma cells are treated with carboplatin and melphalan of various concentrations from 25 to 400 μM (A). Systemic application of both anticancer drugs diluted in fresh Ringer’s solution induces reduction of the tumor population in zebrafish Carboplatin treatment, (B; Melphalan treatment, C). Scale bar, 100 μm. Quantitative analysis of the tumor response in this model yields stable results (D). Data of 8 zebrafish per group from at least 2 independent experiments. dpi, day(s) post injection; GFP: green fluorescent protein.