Insulin promoted aerobic glycolysis, partly, by upregulating PKM2. Media used by insulin treated (100 nM for 8 hours) or untreated cells was collected for measurement of glucose uptake and lactate production as described in materials and methods section. (A) Insulin promoted glucose uptake and lactate production in a PI3K/mTOR sensitive manner. (B) PKM2 knockdown efficiency as checked by real time and Western blotting. (C) Glucose uptake and lactate production decreased on silencing PKM2 in PTEN negative PC3 cells; indicating that insulin stimulated aerobic glycolysis, partly, through PKM2 up-regulation. (D) Cellular proliferation decreased on PKM2 knockdown. C-serum starved cells. Data is expressed as mean ± SE. *P ≤ 0.05.