Figure 5From: Insulin enhances metabolic capacities of cancer cells by dual regulation of glycolytic enzyme pyruvate kinase M2Insulin promoted aerobic glycolysis, partly, by upregulating PKM2. Media used by insulin treated (100 nM for 8 hours) or untreated cells was collected for measurement of glucose uptake and lactate production as described in materials and methods section. (A) Insulin promoted glucose uptake and lactate production in a PI3K/mTOR sensitive manner. (B) PKM2 knockdown efficiency as checked by real time and Western blotting. (C) Glucose uptake and lactate production decreased on silencing PKM2 in PTEN negative PC3 cells; indicating that insulin stimulated aerobic glycolysis, partly, through PKM2 up-regulation. (D) Cellular proliferation decreased on PKM2 knockdown. C-serum starved cells. Data is expressed as mean ± SE. *P ≤ 0.05.Back to article page