Figure 1From: Role of moesin in hyaluronan induced cell migration in glioblastoma multiformeVerification of moesin and CD44 overexpression in GBMs. (A) Western blot analysis was carried out using specifics antibodies for moesin, CD44, S100A11, 14-3-3ζ and γ-enolase and β-actin as described in Materials and Methods. Panel (i) shows increased expression of moesin in GBM tissues (GBM1, GBM2 & GBM3) in comparison to non-malignant brain tissues (NB1 & NB4); (ii) CD44 overexpression in GBMs (GBM1 & GBM3) as compared to non-malignant brain tissues (NB1 & NB4); (iii) S100A11 overexpression in all GBM tissues (GBM1, GBM2 & GBM3) in comparison to non-malignant brain tissues (NB1 & NB4), whereas panels (iv) and (v) and (v) showed downregulation of γ-enolase and 14-3-3ζ respectively in in GBM tissues as compared to non-malignant brain tissues, thereby verifying our iTRAQ results; (iii) β-actin was used as a loading control; (B) Immunohistochemistry (IHC) was performed on independent set paraffin embedded sections of GBM tissues using monoclonal antibodies (Moesin / CD44). IHC analysis showed strong membranous expression of (i) Moesin and (ii) CD44 in glioma cells. Arrows shows membranous expression of moesin / CD44 in GBM tissue sections (Original magnification X400).Back to article page