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Figure 1 | Molecular Cancer

Figure 1

From: Role of moesin in hyaluronan induced cell migration in glioblastoma multiforme

Figure 1

Verification of moesin and CD44 overexpression in GBMs. (A) Western blot analysis was carried out using specifics antibodies for moesin, CD44, S100A11, 14-3-3ζ and γ-enolase and β-actin as described in Materials and Methods. Panel (i) shows increased expression of moesin in GBM tissues (GBM1, GBM2 & GBM3) in comparison to non-malignant brain tissues (NB1 & NB4); (ii) CD44 overexpression in GBMs (GBM1 & GBM3) as compared to non-malignant brain tissues (NB1 & NB4); (iii) S100A11 overexpression in all GBM tissues (GBM1, GBM2 & GBM3) in comparison to non-malignant brain tissues (NB1 & NB4), whereas panels (iv) and (v) and (v) showed downregulation of γ-enolase and 14-3-3ζ respectively in in GBM tissues as compared to non-malignant brain tissues, thereby verifying our iTRAQ results; (iii) β-actin was used as a loading control; (B) Immunohistochemistry (IHC) was performed on independent set paraffin embedded sections of GBM tissues using monoclonal antibodies (Moesin / CD44). IHC analysis showed strong membranous expression of (i) Moesin and (ii) CD44 in glioma cells. Arrows shows membranous expression of moesin / CD44 in GBM tissue sections (Original magnification X400).

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