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Figure 6 | Molecular Cancer

Figure 6

From: Lyn, a Src family kinase, regulates activation of epidermal growth factor receptors in lung adenocarcinoma cells

Figure 6

Immunoprecipitations identify scaffolding and regulatory proteins pulled down with Lyn, PKCß, and EGFR. (A) Anti-phospho-Src (Y-416), Lyn, RACK1 and Cbp/PAG co-immunoprecipitate each other. Immunoprecipitates from unstimulated, serum starved Calu3 cell lysates were performed with antibodies to Cbp/PAG, phospho-Src (Y-416), Lyn, Fyn and RACK1. Western blots were probed with anti-Lyn, and anti-RACK. No Lyn and minimally detectable quantities of RACK1 were immunoprecipitated by anti-Fyn. (B) Cbp/PAG was associated with phosphorylated Src, Lyn and RACK1 in Calu3 while anti-Fyn pulled down Cbp\PAG only in H1975 cells. Western blots of immunoprecipitates from unstimulated, serum starved Calu3 and H1975 cell lysates were probed with anti-Cbp/PAG. (C) EGFR, PKCα or β, Cbp/PAG, and RACK1 are all pulled down with anti-Lyn. Immunoprecipitates with antibodies to Cbp/PAG, Lyn, Fyn, RACK1, ErbB3 and phosphorylated-c-Met from Calu3 lysates were probed in Western blots with antibodies to EGFR and phospho-PKCα,ß.

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Molecular Cancer

ISSN: 1476-4598