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Figure 4 | Molecular Cancer

Figure 4

From: The regulation of toll-like receptor 2 by miR-143 suppresses the invasion and migration of a subset of human colorectal carcinoma cells

Figure 4

miR-143 directly targets TLR2. (A) Expression of TLR2, TLR3, TLR4, and TLR5 was measured in SW620 and HCT116 cells transfected with miR-143. NC is a double-stranded RNA and represents the miRNA mimic negative control. (B) Expression of TLR2 was measured in SW620 and HCT116 cells transfected with Anti-miR-143. NC is a single-stranded RNA and represents the anti-miRNA negative control. (C) The levels of TLR2 mRNA are negatively regulated by miR-143 in SW620 and HCT116 cells. (D) The sequence alignment of miR-143 with the putative binding site in the 3' UTR of the TLR2 gene. Reporter assay in SW620 cells transfected with luciferase constructs (UTR-WT or UTR-MUT) (mean ± SD). (E) Inhibition of migration and invasion of cancer cells by miR-143 was measured using Transwell chambers untreated with matrigel and matrigel-treated Transwell chambers. (WT: wild type; MUT: mutant; **p < 0.01; ***p < 0.001).

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Molecular Cancer

ISSN: 1476-4598