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Figure 5 | Molecular Cancer

Figure 5

From: The regulation of toll-like receptor 2 by miR-143 suppresses the invasion and migration of a subset of human colorectal carcinoma cells

Figure 5

TLR2 modulation accounts for the antimetastatic effect of miR-143. (A) Western blot showing TLR2 expression in SW620 cells 48 h after transfection with the artificial TLR2 expression vector (TLR2). (B) The effects of miR-143, TLR2, and miR-143 combined with TLR2 on the migration and invasion of SW620 cells. (C) Western blot showing TLR2 translation in HCT116 cells 48 hours after transfection with TLR2. (D) The effects of miR-143, TLR2, and miR-143 combined with TLR2 on the migration and invasion of HCT116 cells. (E) Aliquots of 1 ×106 stable miR-143 or miR-NC over-expressing SW620 cells were injected into immunodeficient mice and evaluated for lung colonisation capacity in tail-vein assays. Lung colonisation was measured using bioluminescence at 1, 2, 3, 4, 5 and 6 weeks after injection. (F) The bar graph represents 24 hour time-point measurements of the normalised photon flux from animals injected with SW620 cells. Representative images are shown. n = 5 for each group; error bars indicate SD.

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