Figure 1

Effect of TNFα (25 ng/ml) and IL-17 (50 ng/ml) on glucose utilization and lactate production of human colorectal cancer cell lines. Colorectal cancer cell lines (A, HT-29; B, HCT116; C, T84, and D, Caco-2) were treated for 24 h with cytokines as shown. The medium was harvested and assayed for D-glucose. Glucose utilization was expressed as percentage decrease of glucose in the medium. E. Production of L-lactate by HT-29 cells. Cells were treated with cytokines as shown. The medium was harvested and assayed for L-lactate. F. Effect of EGFR-selective tyrosine protein kinase inhibitor AG1478 on cytokine-stimulated glucose utilization by HT-29 cells. Cells were treated for 24 h with cytokines as shown, with or without AG1478 (1 μM). Culture medium samples were then assayed for D-glucose as in panels A-D. G. Effect of TNFα plus IL-17 on HT-29 cell numbers. Cultures were treated with vehicle or cytokines for 24 h, and cells were then harvested and counted. In panels A-F, each bar represents the mean ± SE of three replicate cultures; in panel G each bar represents the mean ± SE of six replicate cultures. Means with different letters were significantly different, P < 0.05. In panel G, n.s. = not significant based on t-test.