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Figure 2 | Molecular Cancer

Figure 2

From: TNFα and IL-17 cooperatively stimulate glucose metabolism and growth factor production in human colorectal cancer cells

Figure 2

Effect of TNFα and IL-17 on HIF-1α and c-myc expression in HT-29 cells. Duplicate serum-free cultures of HT-29 cells were treated for 4 h with TNFα (25 ng/ml) and/or IL-17 (50 ng/ml), or vehicle (control cultures), as indicated. Protein extract samples (60 μg of cellular protein/lane) were run in 7.5% gels. (A). Western blots were prepared and probed for HIF-1α, c-myc, or α-tubulin. (B). Results were quantified by image analysis. Each bar represents the mean of results obtained for duplicate cultures; the error brackets indicate the range for the duplicates.

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Molecular Cancer

ISSN: 1476-4598