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Figure 4 | Molecular Cancer

Figure 4

From: TNFα and IL-17 cooperatively stimulate glucose metabolism and growth factor production in human colorectal cancer cells

Figure 4

Effect of TNFα and IL-17 on expression of glycolytic pathway components. Duplicate serum-free cultures of HT-29 cells were treated for 12 h or 24 h with TNFα (25 ng/ml) and/or IL-17 (50 ng/ml), or vehicle (control cultures), as indicated. Protein extract samples (60 μg of cellular protein/lane) were run in 7.5% gels. (A). Western blots were prepared with extracts of cells treated for 12 h and probed for SLC2A1, HK2, SLC2A3, LDHA, PKM2, ENO1, PFKFB3, and α-tubulin. (B). Results for SLC2A1 and HK2 were quantified by image analysis. Each bar represents the mean of results obtained for duplicate cultures; the error brackets indicate the range for the duplicates. (C). Western blots were prepared for HT-29 cells treated for 24 h, and the results for SLC2A1 and HK2 were quantified by image analysis. Each bar represents the mean of results obtained for duplicate cultures; the error brackets indicate the range for the duplicates.

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