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Figure 6 | Molecular Cancer

Figure 6

From: TNFα and IL-17 cooperatively stimulate glucose metabolism and growth factor production in human colorectal cancer cells

Figure 6

Inhibition by TPCA-1 of cytokine-stimulated lactate production and expression of SLC2A1 and HK2 by HT-29 cells. Serum-free cultures of HT-29 cells were treated for 12 h with TNFα (25 ng/ml) + IL-17 (50 ng/ml), in the presence of TPCA-1 or vehicle, as shown. Medium samples were then collected for lactate assays, and protein extract samples of cell monolayers (30 μg of cellular protein/lane) were run in 7.5% gels. (A). Effect of increasing concentrations of TPCA-1 on lactate production. Analysis of the data by linear regression showed a significant dose-dependent inhibition of lactate production by TPCA-1, P = 0.006. (B). Western blots of cell extract proteins were probed for SLC2A1, HK2, or α-tubulin. (C). Results of Western blots shown in panel B were quantified by image analysis. Analysis of the data by linear regression showed significant dose-dependent inhibition by TPCA-1 of HK-2 (P = 0.005) but not SLC2A1 (P = 0.84, n.s.) expression.

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