Figure 7

Medium conditioned by HT-29 cells treated with TNFα and IL-17 stimulates growth/survival of NIL8 cells. (A). NIL8 cells were treated for 24 h with nothing, or 10 μl unconditioned medium (open bars), or insulin (1 μg/ml) (filled gray bar), or 10 μl of medium conditioned by cultures of HT-29 cells treated with vehicle (control), TNFα, IL-17, or TNFα + IL-17 (filled black bars). Cell growth/survival of the NIL8 cells was then assayed (Methods). Each bar is the mean of quadruplicate assays ± SE. Means with different letters were significantly different, P < 0.05. (B). Cell growth/survival of NIL8 cells treated with: Bar 1: unconditioned McCoys 5A medium; Bar 2: medium conditioned by HT-29 cells stimulated with 25 ng/ml TNFα + 50 ng/ml IL-17 for 24 h; Bar 3: unconditioned medium containing 25 ng/ml TNFα + 50 ng/ml IL-17. Means with different letters were significantly different, P < 0.05. (C). NIL8 cells were treated for 24 h with EGF (300 ng/ml) (○), insulin (10 μg/ml) (■), or 10 μl of medium conditioned by HT-29 cells stimulated with TNFα + IL-17 (▲), plus increasing concentrations of AG1478. Cell growth/survival vs. unstimulated control NIL8 cells was then assayed (Methods). Data were plotted as % growth stimulation observed in the absence of AG1478. Results show the means of quadruplicate assays ± SE.