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Table 1 Summary of TNF-α PCR-RFLP analysis

From: Association of tumor necrosis factor-alpha promoter variants with risk of HPV-associated oral squamous cell carcinoma

Variants

Primer sequence (5′ to 3′)

PCR products

Anneal T

 

Allele

 

Upper-forward; lower-reverse

 

(°C)

Enzymes used

determination

NF-α-308G > A (rs1800629)

AGGCAATAGCTTTTGAGGGCCATG

80 bp

60

Sty I

Intact

:-308GG

TCTTCTGGGCCACTGACTGAT

58 bp + 22 bp

:-308GA + AA

TNF-α-857C > T (rs1799724)

CGAGTATGGGGACCCTCCCATTAA

100 bp

57

Ase1

Intact

:-857CC

ACGTCCCCTGTATTCCATACC

78 bp + 22 bp

:-857CT + TT

TNF-α-863C > A (rs1800630)

GACCACAGCAATGGGTAGGAGA

86 bp

60

Bsl I

Intact

:-863CC

TCTACATGGCCCTGTCCTCGT

64 bp + 22 bp

:-863CA + AA

TNF-α-1031T > C (rs1799964)

AGAGCTGTGGGGAGAACAAA

247 bp

62

Bbs I

Intact

:-1031TT

CTCCTACCCATTGCTGTGGT

     

127 bp + 120 bp

:-1031TC + CC