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Table 1 FGFR3 impact in pancreatic tumour cell lines

From: FGFR3 has tumor suppressor properties in cells with epithelial phenotype

  Epithelial-like   Mesenchymal-like
   Capan-2    BxPC-3     Mia PaCa-2    PANC-1  
Colony formation (Ratio/CT) Colony area (μm2) Migration (number of cells) Senescent cells (%) Tumour mass (mg) Colony formation (Ratio/CT) Colony area (mm2) Tumour mass (mg) Colony formation (Ratio/CT) Colony area (mm2) Migration (number of cells) Senescent cells (%) Tumour mass (mg) Colony formation (Ratio/CT) Colony area (mm2) Tumour mass (mg)
CTRL 1 ± 0.13 19.3 ± 5.0 997 ± 6 24.4 ± 5.3 203 ± 37 1 ± 0.18 24.2 ± 15.1 42 ± 0.01 1 ± 0.004 13.8 ± 4.7 98 ± 2 8.6 ± 1.9 21 ± 3 1 ± 0.05 9.9 ± 9.2 59 ± 39
FGFR3-IIIb 1.38 ± 0.06* 9.1 ± 1.5* 1524 ± 26*** 35.7 ± 3.9** 110 ± 12* 0.97 ± 0.10 15.9 ± 11.9** 25 ±0.01* 1.52 ± 0.18** 17.0 ± 4.6** 346 ± 9*** 4.7 ± 1.9*** 292 ± 133* 1.28 ± 0.04* 21.5 ± 14.1*** 137 ± 51*
FGFR3-IIIc 1.52 ± 0.27* 6.3 ± 1.5** 2050 ± 18*** 32.4 ± 5.5* 111 ± 14* 1.37 ± 0.04* 12.7 ± 9.7*** 23 ± 0.01* 1.28 ± 0.17* 23.7 ± 8.1*** 515 ± 22*** 5.0 ± 1*** 367 ± 113** 1.23 ± 0.04* 22.8 ± 14.6*** 138 ± 39*
FGFR3-IIIc-KD 1.21 ± 0.05* 15.7 ± 11.1 1492 ± 10*** 16.9 ± 3.26* 212 ± 71 1.26 ± 0.04** 20.9 ± 14.2 ND ND ND ND ND ND 1.55 ± 0.12* 15.1 ± 8.7** ND
  1. Cells were transduced to obtain 70% of transduced cells as analyzed by flow cytometry. For colony formation assays, 500 cells were plated in 10 mm dished in triplicates. Dishes were processed for violet crystal staining and blind counted. Colony areas were measured with Image J software. Senescence was detected as indicated in the methods section. Senescence was not detectable in BxPC-3 and PANC-1 cells. Results are reported as means ± SD except for tumour masses ± SEM. *: p < 0.05 **: p < 0.01 ***: p < 0.001 as compared to control. Ratio/CT: results are expressed as ratio over the control condition. ND: not done.