Figure 1

Analysis of m Parm-1 gene expression in sorted lymphoid leukemia samples. Semi-quantitative RT-PCR analysis of Parm-1 in 5 B and 5 T leukemias : (B4, Cd45⁺Cd19⁺Sca1⁺; B5, Cd45R⁺Cd19⁺Sca1⁺; B6, Cd45R⁺Cd19⁺Sca1⁺; B7, Cd45R⁺Cd19⁺Sca1⁺; B8, Cd45R⁺Cd19⁺Sca1⁺) (T4, Cd4⁺Cd8⁺; T5, Cd4⁺Cd8⁺; T6, Cd4⁺Cd8⁺; T7, Cd4⁺Cd8⁺; T8, Cd4⁺Cd8^-). RT-PCRs were performed in triplicate using the following actin (forward primer: 5’- tgacggggtcacccacactgtgcccatcta-3’, reverse primer: 5’-ctagaagcatttgcggtggacgatggaggg-3’) and murine Parm-1 (forward primer: 5’- gttagctgttttggggacca-3’, reverse primer: 5’-cgtgcaaattagcatctgga-3’) specific primers. PCR products were analyzed on agarose gel and band density was quantified with Quantity One Image Software. The actin gene was used as internal control and expression levels in each leukemia are presented as a gene/actin density ratio. Data are representative of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance, and P lower to 0.05 was considered to be significant (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001) compared with their respective control (CB2, B cells from normal spleen and CT2, T cells from normal thymus).