Overexpression of Akt1 results in increased phosphorylation of Akt, CXCR4 expression, and proliferation. A) DU145 cells were stably transfected with HA-tagged Akt1. Lysate was collected from serum-starved cells, and protein levels were analyzed by Western blot. B) Cells were cultured in the presence or absence of serum, lysate was collected, and protein levels were analyzed by Western blot. C) Proliferation of DU145-Neo and DU145-HA-Akt1 cells was determined over three days. D) Cell cycle analysis of DU145-Neo and DU145-HA-Akt1 cells was performed. E) DU145-Neo or DU145-HA-Akt1 cells treated with 5 μM AMD3100 for two hours were plated on Matrigel coated inserts, allowed to invade for 24 hours, and stained with Crystal Violet. Total number of migrated cells was counted under 10X magnification in five fields. *: p < 0.01.