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Figure 5 | Molecular Cancer

Figure 5

From: Epigenetic silencing of RASSF1A deregulates cytoskeleton and promotes malignant behavior of adrenocortical carcinoma

Figure 5

Constitutive expression of RASSF1A reduces the invasive, migratory and clonogenic potentials of SW-13 cells. (A), SW-13/A and SW-13/AM cells were allowed to invade through Matrigel from upper chambers containing serum-free medium to lower chambers containing 10% FBS medium. After 24 hours, and invaded cells were fixed, stained with crystal violet and tabulated. Data represent results from one of two independent experiments with similar results. (B) SW-13/V, SW-13/A and SW-13/AM cells were allowed to migrate through modified Boyden Chambers (8 μM pore size) for 4 hours and migrated cells to the lower side of the membrane were fixed, stained with crystal violet and tabulated. Data from a representative experiment of triplicate experiments with similar results are shown. (C) SW-13/V, SW-13/A and SW-13/AM cells were seeded in 6-well plates in low densities (5000 cells/well) and allowed to grow for 7 days in G-418 containing medium. Cells were washed with PBS, fixed in 3.7 % formaldehyde solution stained with crystal violet and colonies with 10 +/− 2 cells were counted and averaged from 6 wells. Data from a representative experiment of quadruplicate experiments with similar results are shown.

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