Combined treatment of FTI-277 and IPA3, but not FTI-277 alone, increases the cell size of HeLa and A375MM cells. HeLa and A375MM cells were treated for 48 h as indicated in Figures 2, 3 and in Methods, and stained with relevant antibodies. Olympus ScanR analysis software was used to calculate the area of each cell counted. The graph represents the relative area (%) of cells treated as indicated for HeLa and A375MM cells (respective panels) with cells treated with vehicle, arbitrarily set at 100%. The graph shows the mean ± SD of 2 independent experiments, each run in triplicate (three well per condition). More than 73 cells for A375MM and 573 cells for HeLa cells were counted per sample in each experiment. Results of t-test are shown above the graph: ns: no significant deviation from vehicle, p-value >0.05; * p-value <0.05; nn: no significant deviation from FTI-277, p-value >0.05.