Epigenetic enzymatic activities in response to dietary GE and/or TAM treatment in mice breast tumors. Left panel, MDA-MB-231 breast tumor xenografts (Protocol 1); right panel, C3(1)-SV40 Tag transgenic mouse tumors (Protocol 2). GE and/or TAM treatment were described above. A) HDACs enzymatic activity. B) DNMTs enzymatic activity. Nuclear proteins from mice breast tumors were extracted as described previously. The HDACs and DNMTs activity assays were performed according to the manufacturer’s protocols. The values of enzymatic activities of HDACs and DNMTs are the means of three independent experiments from all tumors per group. Columns, mean; Bars, SD. *, P < 0.05, significantly different from control; * * P< 0.01, significantly different from control.