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Figure 5 | Molecular Cancer

Figure 5

From: Histone acetyltransferase PCAF Up-regulated cell apoptosis in hepatocellular carcinoma via acetylating histone H4 and inactivating AKT signaling

Figure 5

Forced expression of PCAF inhibited the growth of HCC xenografts, up-regulated histone H4 acetylation, suppressed phosphorylation of AKT, and accelerated cell apoptosis. (A) IHC experiments was performed to verify the positive expression of PCAF in the xenografts from Huh7 PCAF cells (a) and the negative staining of PCAF in the xenografts from Huh7 Control cells (b). The PCAF staining was located in cell nucleus primarily indicated by black arrow. There was more acetylated histone H4 expressing in the xenografts from Huh7 PCAF cells (c) than the xenografts from Huh7 PCAF cells (d). The acetylated histone H4 was found mainly in cell nucleus (black arrow). The phosphorylation of AKT protein was upregulated in the xenografts from Huh7 PCAF cells (e), compared to the xenografts from Huh7 Control cells (f). The phospho-AKT expression was detected in both cell nucleus (black arrow) and cytoplasm (white arrow); (B) Xenografts from Huh7 PCAF cells grew significantly slower than xenografts from Huh7 Control cells; (C) There were more apoptotic cells in the xenograft tissues from Huh7 PCAF cells (a) than the xenograft tissues from Huh7 Control cells (b).

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