L-plastin knock-down reduces metastasis of prostate carcinoma cells. Tumor metastasis monitored by in vivo bioluminescence imaging. (A) Representative examples of dorsal optical images of mice injected with PC3M, PC3M nt shRNA or PC3M LPL shRNA cells at the indicated time points are shown. (B) Quantification of metastasis. Numbers of single spots, representing metastatic lesions, were counted from a ventral and dorsal view per animal and plotted over time after cancer cell injection. Results were analyzed using student’s t-test, data represent means ± SEM (n = 12 for PC3M and PC3M nt shRNA; n = 17 for PC3M LPL shRNA; * = p < 0.05 for PC3M vs. PC3M LPL shRNA and PC3M nt shRNA vs. PC3M LPL shRNA).