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Figure 6 | Molecular Cancer

Figure 6

From: Maternal embryonic leucine zipper kinase enhances gastric cancer progression via the FAK/Paxillin pathway

Figure 6

MELK specific inhibitor OTSSP167 suppresses cell migration and invasion. A, Immunoblotting representing MELK expression in SGC7901 cell following OTSSP167 treatment for indicated period. In the upper panel, SGC7901 cell were treated with different concentration (0, 0.1, 1 μM) of OTSSP167 for 1 h and 2 h. In the lower panel, SGC7901/vector and SGC7901/MELK cell were treated with 1 μM OTSSP167 for 2 h. B, OTSSP167 suppresses SGC7901 cell migration and invasion measured by a transwell chamber assay (**P < 0.01). C and D, OTSSP167 partially reverses the up-regulation of pY397, pY576/577, and pY925 of FAK, and pY118 of paxillin caused by MELK overexpression. SGC7901/vector and SGC7901/MELK cells were treated with 1 μM OTSSP167 for indicated period and then were collected and measured by immunoblotting. E, Effect of OTSSP167 on SGC7901 cell migration and invasion. SGC7901/vector and SGC7901/MELK cells were treated with OTSSP167 (1 μM); cell migration and invasion were measured after 2 h.

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Molecular Cancer

ISSN: 1476-4598