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Figure 2 | Molecular Cancer

Figure 2

From: Acylglycerol kinase promotes cell proliferation and tumorigenicity in breast cancer via suppression of the FOXO1 transcription factor

Figure 2

AGK plays a key role in breast cancer cell proliferation and tumorigenicity. (A) AGK expression levels significantly correlated with Ki-67 expression in human breast cancer tissues (n = 203; P < 0.001). Two representative cases are shown (left) and percentage of specimens with low or high AGK expression, relative to the levels of Ki-67 staining (right). (B) Western blotting analysis of AGK expression in AGK-infected MCF-7 and SKBR3 cells (upper panel) and AGK-silenced MCF-7 and SKBR3 cells (lower panel). GAPDH was used as a loading control. (C-F) MTT assay (C, E) and colony formation assay (D, F) indicated that the growth rate increased in AGK-overexpressing cells and decreased in AGK-silenced cells. The absorbance at day 1–5 was normalized to the absorbance at day 0 used as control (100%). The number of colonies was quantified in the colony formation assay. Each bar represents the mean ± SD of three independent experiments. *P < 0.05.

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