Skip to main content
Figure 1 | Molecular Cancer

Figure 1

From: Survivin safeguards chromosome numbers and protects from aneuploidy independently from p53

Figure 1

Knockdown of Survivin leads to polyploidy in p53-positive tumor cells and isogenic cell lines deficient for p53. HCT116, SBC-2, U87-MG and isogenic HCT116p53−/−, SBC-2shp53, and U87-MGshp53 cells were transduced with shRNAs targeting Survivin (shSurv) or Luciferase (shLuc). a: Representative indirect-immunofluorescence images of transduced SBC-2 cells. The shLuc-transduced cell shows a normal bipolar spindle formation in anaphase. The shSurv-transduced cell shows multipolar spindles and increased numbers of chromosomes. Microtubules (green), membranes (red) and chromosomes (blue). Magnification bars: 10 μm. b: FACS-analyses of cells with knockdown of Survivin depicting the appearance of polyploid cells in wild type and p53-deficient cells. c: Quantitative analysis of the polyploidy phenotype (i.e. cells with DNA contents >4n or >6n for SBC-2) of HCT116, U87-MG and SBC-2 and corresponding p53-negative cells. Data represents mean values and standard error of the mean (SEM). d: Western blot analysis of whole cell lysates demonstrating knockdown of Survivin and accumulation of p53 in Survivin-depleted HCT116, U87-MG and SBC-2 wild type cells but not in controls (shLuc) or p53-deficient cells.

Back to article page