Figure 2
From: Survivin safeguards chromosome numbers and protects from aneuploidy independently from p53
Accumulating DNA contents after knockdown of Survivin leads to transient cell cycle arrest in p53-positive tumor cells. a: Western blot analysis of U87-MG glioma cells and U87-MGshp53 72 h after transduction of a shRNA targeting Survivin demonstrating an increase in steady state protein levels of p53, p21waf/cip. The increase in Cyclin D1 and Cyclin E protein levels in p53-positive wild type cells indicate a cell cycle arrest. A p21waf/cip–mediated cell cycle arrest is not observed in shLuc-transduced control cells or in isogenic U87-MGshp53 cells with knockdown of Survivin. Red values depict densitometric estimation of protein levels, normalized to Tubulin. b: Western blot analysis and parallel DNA-analysis of HCT116 cells at different time points after RNAi showing a gradual increase in p53, p21waf/cip, Cyclin D1 and Cyclin E protein levels only in p53-positive cells (wt) after knockdown of Survivin but not in corresponding HCT116p53−/− cells and shLuc controls. c: DNA analyses of HCT116 and HCT116p53−/− after transduction showing time-dependent gradual increase in polyploidy in Survivin-depleted cells. The amount of DNA content (n) in the cell fractions is indicated.