Figure 4
From: Survivin safeguards chromosome numbers and protects from aneuploidy independently from p53
Survivin controls kinetochore-microtubuli attachments. a: Indirect-immunofluorescence images of mitotic SBC-2 wild type cells, transduced with shLuc or shSurv, respectively. The shLuc-transduced cell shows a normal bipolar spindle assembly during anaphase. The depicted shSurv-SBC-2 cell is dodecaploid and displays abnormal spindle formation in metaphase with a poly-merotelic connected chromosome (arrowhead; inset). b: Different angles of a 3D-reconstructed z-stack of the same merotelic attachment shown in a: demonstrating binding of up to four microtubli from three different directions to a single kinetochore (KTa). MT, microtubuli (red); KT, kinetochore; (yellow); membrane (green).