Survivin protects cells from chromosomal instability. a: SKY-Analysis reveals chromosomal instability (gain of numerical and structural chromosomal aberrations as well as polyploidy) in Survivin-depleted HCT116p53−/− cells 72 h after retroviral transduction. Representative karyogram of shLuc-transduced HCT116p53−/− cell with hypodiploid (2n-) karyotype 45,X,-Y,t(5;7)(q13;pter), der(10)dup(10)(q23.1q26.1)t(10;16)(q26.1;q23), der(16)t(8;16)(q13;pter), der(18) t(17;18)(q21.3;pter) (arrowheads). b: Exemplary karyograms of Survivin-depleted HCT116p53−/− cells showing nearly tetraploid karyotypes (87,XX), harboring the aberrations found in shLuc, but also additional aberrations. Upper karyogram: additional dicentric chromosome 1 (arrow) and loss of two chromosomes 2. Lower karyogram: additional structural aberrations del(2)(q13), der(9)t(9;12), der(15)t(15;17), der(20)t(3;20) and loss of chromosome 13, 19, 21 (arrows). c: DAPI-stained metaphase of HCT116p53−/− cells with knockdown of Survivin showing a hypohexadecaploid (16n-) karyotype (360 chromosomes) with dicentric chromosomes (arrows) and chromosome breaks (arrowheads).