Figure 3
From: DUSP3/VHR is a pro-angiogenic atypical dual-specificity phosphatase
DUSP3 downregulation affects HUVEC spheroids sprouting. (Ai) Western blot and quantification of DUSP3 protein expression in siCTL, siDUSP3-1 and siDUSP3-2 transfection conditions represented as a ratio of DUSP3 on GAPDH. (Aii) Representative images of spheroid sprouting assay performed with endothelial cells as indicated with siCTL, siDUSP3-1 and siDUSP3-2 in the presence of PMA (75 ng/mL) or b-FGF (10 ng/mL). (Aiii) The mean cumulative number of sprouts per spheroid was assessed after 48 hours. Results are presented as mean ± SEM. *, P < 0,5; **, P < 0,01.