Ex-vivo microvasculature outgrowth from DUSP3+/+and DUSP3−/−mice aortic rings. (A) Phase contrast micrographs of thoracic aortas from 17 weeks old DUSP3+/+ and DUSP3−/− mice grown for 9 days in collagen additive-free (no stimulation), in 2.5% serum containing collagen gels or in 20 ng/mL of b-FGF-supplemented collagen gels. Magnification ×25. (B) Computerized quantification of number of microvessel intersections and maximal length of vessels from culture conditions shown in A. X axis represents the length of the aortic microvessel outgrowth and Y-axis represents the number of intersections of the microvessels. The arrows in the b-FGF stimulated conditions indicates the maximal vessel growth, Lmax (mm) for DUSP3−/− and for DUSP3+/+ aortas. ***p <0.001 (t-student test).