Expression of Fbxw7 and its clinical significance in HCC cases. A) Representative western blot analysis of Fbxw7 expression in cancer (T) and matched noncancerous tissues (NT) was shown. Quantification of the data revealed that Fbxw7 protein expression in HCC tissues was significantly lower than that in the normal tumor-adjacent tissues. n = 20; Values are depicted as Mean ± SEM; **P < 0.01 by t test. B) Kaplan–Meier overall 5-year and C) disease-free survival curves for HCC patients according to their Fbxw7 protein expression status. The Fbxw7 positive expression group (n = 24), IHC score of Fbxw7 = 1–3; Fbxw7 negative expression group (n = 36), IHC score of Fbxw7 = 0; *P < 0.05 by log-rank test.