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Figure 2 | Molecular Cancer

Figure 2

From: Fbxw7 is an independent prognostic marker and induces apoptosis and growth arrest by regulating YAP abundance in hepatocellular carcinoma

Figure 2

Fbxw7 regulates apoptosis and proliferation in HCC cells. A) HepG2 and Hep3B cells that had been transfected with Flag-Fbxw7 and Fbxw7-shRNA, respectively, were subjected to western blotting for Fbxw7. The data are representative of multiple repeats with similar results. B) Quantification of the apoptotic cell population by flow cytometry. Fbxw7 overexpressing HepG2 cells were composed of a larger subset of apoptotic cells compared with the control cells and Fbxw7 knockdown decreased the percentage of apoptotic Hep3B cells. **P < 0.01 by t test; n = 3 repeats with similar results. C) The activity of the pro-apoptotic caspases 3 and 7 was up-regulated after Fbxw7 overexpression in HepG2 cells and down-regulated after Fbxw7 knockdown in Hep3B cells. **P < 0.01 by t test; n = 3 repeats with similar results. D) Cell proliferation as measured by BrdU incorporation was inhibited by Fbxw7 overexpression in HepG2 cells and promoted by Fbxw7 knockdown in Hep3B cells. **P < 0.01 by t test; n = 3 repeats with similar results. E) As assessed by MTT assays, Fbxw7 overexpression was found to reduce the viability of HepG2 cells and Fbxw7 knockdown was found to enhance the viability of Hep3B cells. **P < 0.01 by two-way ANOVA; n = 3 repeats with similar results. Values are depicted as Mean ± SEM.

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