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Figure 6 | Molecular Cancer

Figure 6

From: Fbxw7 is an independent prognostic marker and induces apoptosis and growth arrest by regulating YAP abundance in hepatocellular carcinoma

Figure 6

Fbxw7’s suppression of Hep3B cell growth was partially reverted by YAP. A) Flag-Fbxw7 transfected Hep3B cells successfully up-regulated Fbxw7 protein expression as shown by western blot. Fbxw7 overexpression in the same cell line could reduce the levels of YAP. Fbxw7 over-expressing cells that were transfected with HA-YAP partially rescued the phenotype, showing higher YAP levels. The data are representative of multiple repeats with similar results. B) Apoptotic cells were measured by flow cytometry. Restoring YAP expression decreased the percentage of apoptotic cells in Flag-Fbxw7 transfected Hep3B cells. **P < 0.01 by one-way ANOVA; n = 3 repeats with similar results. C) The activity of the pro-apoptotic caspases 3 and 7 in Fbxw7 overexpressing Hep3B cells was decreased by HA-YAP transfection. **P < 0.01 by one-way ANOVA; n = 3 repeats with similar results. D) A BrdU assay showed that YAP promotes proliferation in Fbxw7 overexpressing Hep3B cells. **P < 0.01 by one-way ANOVA; n = 3 repeats with similar results. E) YAP was found to enhance the viability of Fbxw7 over-expressing Hep3B cells (MTT assay). **P < 0.01 by two-way ANOVA; n = 3 repeats with similar results. Values are depicted as the Mean ± SEM.

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