Figure 5
Integrin β3 silencing and inhibition of stromal TGF-β improves survival and attenuates tumor cell growth in an orthotopic mouse model of lung carcinoma. (A) Kaplan-Meier survival curves for mice injected with β3 integrin-deficient or β3 integrin-competent cells that were pretreated with or without TGF-β. Mice were injected intraperitoneally with 200 μg of the TGF-β inhibitor peptide P144 as described in Materials and methods. (B) Tumor size determined from microphotographs obtained from tumor sections and expressed in cm2 (maximum diameter). Image analyses were performed using fiji software. Data were analyzed using a one-way ANOVA followed by a Dunnett’s multiple comparison test (** p < 0.001: H157 TGF-β PBS vs H157 control PBS; §§p = 0.001: shRNA β3 TGF-β PBS vs H157 control PBS; ##p = 0.004: H157 TGF-β PBS vs H157 TGF-β P144). (C) Micro-CT analyses of mouse lungs before sacrifice. A representative image from each group is shown.