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Figure 7 | Molecular Cancer

Figure 7

From: Loss of miR-638 in vitro promotes cell invasion and a mesenchymal-like transition by influencing SOX2 expression in colorectal carcinoma cells

Figure 7

SOX2 overexpression rescues the miR-638-induced transition to epithelial-like cells. A) SOX2 overexpression rescued the miR-638-induced transition to epithelial-like cells, as verified by the level of expression of the epithelial cell marker ZO-1/E-cadherin and the mesenchymal cell marker vimentin by Western blotting. SOX2 overexpression was verified by Western blotting using an anti-Myc antibody. SOX2 overexpression in miR-638-transfected SW1116 cells decreased the expression of the epithelial cell marker ZO-1/E-cadherin and increased the expression of the mesenchymal cell marker vimentin. B) SOX2 overexpression rescued the miR-638-induced transition to epithelial-like cells, as verified by the cell morphology change. SOX2 overexpression in miR-638-transfected SW1116 cells decreased cell-to-cell contacts and promoted lamellipodium stretching. Cells were stained for F-actin (red). Nuclei were counterstained with DAPI (blue). Scale bar, 20 μm. SOX2 overexpression rescued the miR-638-induced transition to epithelial-like cells, as verified by the cell motility change. The overexpression of SOX2 reversed the miR-638 inhibition of cell invasion (C) and cell migration (D).

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Molecular Cancer

ISSN: 1476-4598