Figure 1

GAS5 expression is lower in MPM and it is increased upon drug induced growth arrest. A. GAS5 is lower in MPM cell lines compared to normal mesothelial cell lines. GAS5 lncRNA expression was analysed by qRT-PCR in 22 MPM cell lines and in 7 normal mesothelial cell lines. Expression of GAS5 was normalized to internal control histones relative to the mean expression of GAS5 in normal mesothelial cells in culture according to –∆∆Ct method. B and C. Drug induced growth arrest increases GAS5 expression in MPM primary cells (ZL55SPT and SDM103T2) and cell lines (ZL55 and ACC-Meso4). D. Expression of GAS5 promoter reporter gene was analysed in control versus HhAntag and NVP-BEZ235 treated samples. Promoter activity, expressed as relative light unit (RLU) normalized to control set at 100%, is significantly increased by treatment with HhAntag and NVP-BEZ235. Values are expressed as mean ± SD from three independent experiments *; p < 0.05.