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Figure 4 | Molecular Cancer

Figure 4

From: GAS5 long non-coding RNA in malignant pleural mesothelioma

Figure 4

Nuclear enrichment of GAS5 lncRNA after induction of growth arrest by HhAntag. A. Left panel: Representative micrographs of fluorescence in situ hybridization showing GAS5 (yellow), GAPDH (green) for cytosolic control, RNU (red) for nuclear mRNA control, and nuclei stained with DAPI (blue). Right panel: same image as left panel showing only RNU and GAS5. Scale bar: 10 μm. B. Quantification of GAS5 in ZL55SPT cells and nuclei was determined by calculating intensity of fluorescent spots by Imaris image analysis using spot detection method. The graph plots the fluorescent spots per cell vs. intensity. *; p < 0.05 compared to the non-treated control. C. Integration of area under the curve to evaluate the proportion of nuclear GAS5 in control and HhAntag-induced growth arrest. Representative of three independent experiments.

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