Figure 5

GAS5 expression is higher in podoplanin positive MPM tumor cells. A. Fluorescence in situ hybridization and immunohistochemical analysis of GAS5, Ki-67 and podoplanin in a representative patient. Upper panel shows fluorescence in situ hybridization of control and GAS5, immunostaining of Ki-67 (ab15580, Abcam). Lower panel shows the immunostaining of control and podoplanin (D2-40, DAKOCytomation) and hematoxylin and eosin stained tumor tissue. Scale bar: 50 μm. B. Matrix of relative gene expression in epitheliod, biphasic and sarcomatoid mesothelioma shown as heat map. Heat map is a grid of rectangles with colors that indicate the value of the matrix elements, where high expression is blue and low expression is red. Rows of each heat map correspond to genes, whilst columns correspond to MPM tumor samples. C. GAS5 expression is higher in MPM tissue expressing higher levels of podoplanin *p < 0.05, Mann–Whitney test.