Table 1 Primer sequences used in the present study
Gene* | Primers |
|---|---|
GAPDH F | 5′-CATGAGAAGTATGACAACAGCCT-3′ |
GAPDH R | 5′-AGTCCTTCCACGATACCAAAGT-3′ |
miR-300 F | 5′-TATACAAGGGCAGACTCTCTCT-3′ |
U6 F | 5′-CGCAAGGATGACACG CAAATTCGT-3′ |
Twist 3′UTR F | 5′-TCCCCCGGGAAGCAGCTACTGACAGGC-3′ |
Twist 3′UTR R | 5′-CCCAAGCTTGTATGATCTCACCAGGGA-3′ |
Twist qPCR F | 5′-GGACAAGCTGAGCAAGAT-3′ |
Twist qPCR R | 5′-CTCTGGAGGACCTGGTAG-3′ |