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Table 1 Primer sequences used in the present study

From: miR-300 inhibits epithelial to mesenchymal transition and metastasis by targeting Twist in human epithelial cancer

Gene* Primers
GAPDH F 5′-CATGAGAAGTATGACAACAGCCT-3′
GAPDH R 5′-AGTCCTTCCACGATACCAAAGT-3′
miR-300 F 5′-TATACAAGGGCAGACTCTCTCT-3′
U6 F 5′-CGCAAGGATGACACG CAAATTCGT-3′
Twist 3′UTR F 5′-TCCCCCGGGAAGCAGCTACTGACAGGC-3′
Twist 3′UTR R 5′-CCCAAGCTTGTATGATCTCACCAGGGA-3′
Twist qPCR F 5′-GGACAAGCTGAGCAAGAT-3′
Twist qPCR R 5′-CTCTGGAGGACCTGGTAG-3′
  1. *F, forward; R, reverse.