Analysis of PI3K/AKT signaling. A: Sequencing results for PIK3CA A3140G corresponding to the H1047R mutation in the catalytic subunit of PI3 kinase are shown as histograms. B: Phospho-protein and total protein levels for AKT were examined by Western Blotting. C-D: No differential response between wild-type and BRAF-mutant cell clones was found in proliferation assays. E: In Western blot analysis, PI-103 inhibited phosphorylation of AKT residues T-308 and S-473 in a dose-dependent manner in all cell clones.