Expression analysis of DLEU2 and miR-15/16 in paediatric AML. Here we interrogate the gene, miRNA and precursor miRNA expression in paediatric AML compared to non-leukaemic. This interrogation includes DLEU2 and embedded miR-15a/16-1 on chromosome 13q4. The leukaemic group refers to diagnostic bone marrow from paediatric patients. The non-leukaemic group consists of CD sorted cell populations (CD19+, CD33+, CD34+, CD45+) and patient remission specimens, and is represented by the dashed line at Y = 1. Fold Change (FC) is plotted using normalized data and the 2-ΔΔCt method ± SD, and shows the fold change calculated from the means of each group. A. Gene expression including DLEU1, DLEU2 and TRIM13 in paediatric AML (n = 10) compared to non-leukaemic (n = 13) expression. DLEU2 shows a significant down-regulation in AML compared to non-leukaemic expression (0.07 FC, p = 0.014 represented by **), however there is no significant change in expression for TRIM13 or DLEU1. B. Mature microRNA expression, including primary precursor transcript (PRI) and alternate miRNA expression (*), from the miR-15a/16-1 miRNA cluster embedded within DLEU2 for paediatric AML (n = 28, including the 10 used in Figure 2A) compared to non-leukaemic specimens (n = 30, including the 13 used in Figure 2A). Here the miR-15a/16-1 PRI transcript is 3.03-fold higher in expression compared to non-leukaemic expression. Additionally, miR-16-1* (2.52 FC), miR-15a* (2.24 FC) and miR-15a (1.5 FC) also show increases in expression in paediatric AML. No significant change in expression was observed for miR-16 (0.94 FC) in paediatric AML compared to non-leukaemic expression.