Figure 3
From: microRNA-139-5p exerts tumor suppressor function by targeting NOTCH1 in colorectal cancer
miR-139-5p induced colon cancer cell apoptosis and cell cycle arrest at G0/G1 phase. (A) miR-139-5p caused cell cycle arrest at G0/G1 phase in DLD1 and HCT116 cells (left panel). Quantitative analysis of G0/G1 proportion in DLD1 and HCT116, The experiments were repeated three times in triplicate. Data are mean ± SD. (B) G0/G1 phase cell cycle related protein expression including p21Cip1/Waf1, p27Kip1 and p53 were determined by western blot in DLD1 and HCT116 cells (C) miR-139-5p induced apoptosis in DLD1 and HCT116 cells. Cell apoptosis was examined by flow cytometry analysis of Annexin V-APC and 7-AAD double-staining. Data represented the average of the early and late apoptotic cells, respectively. (D) Apoptotic-related protein expression (PARP, caspase-3, 7, 8) were detected by western blot.