DTX3L-mediated cell migration of PC3 cells might also be dependent on STAT3. (A-F) PC3-siSTAT3 single knockdown cells (A), PC3-siDTX3L/siSTAT3 (B), PC3-siSTAT1/siSTAT3 (C) double knockdown cells and PC3-siDTX3L/siSTAT1/siSTAT3 (D) triple knockdown cells were seeded into 6-well plates and treated as described in Material and Methods. At 0, 12, 24, and 36 h photographs were made and quantified as described in Material and Methods. Values represent the mean of three independent experiments and the error bars represent the SE. Statistical analysis was performed using the Student's t test. *P < 0.05, **P < 0.001 and ***P < 0.0001.