Figure 3

Influence of TGFB1 and activin A on cellular growth. The cells were stimulated with the indicated doses of TGFB1 or activin A for 72 hours. Cell proliferation was assayed using an MTT assay. (A) Cellular growth of Sui65 cell line (homozygous deletion of ACVR1B and SMAD4 genes). Both TGFB1 (0.1 ng/mL, P = 0.56; 1 ng/mL, P = 0.51; 10 ng/mL, P = 0.69) and activin A (1 ng/mL, P = 0.51; 10 ng/mL, P = 0.71; 100 ng/mL, P = 0.65) did not influence the cellular growth. (B) Cellular growth of Sui66 cell line (wild- type ACVR1B and SMAD4 genes). Both TGFB1 (0.1 ng/mL, P = 0.0049*; 1 ng/mL, P = 0.0028*; 10 ng/mL, P = 0.0016*) and activin A (1 ng/mL, P = 0.051; 10 ng/mL, P = 0.010*; 100 ng/mL, P = 0.0081*) inhibited cellular growth. (C) Cellular growth of Sui68 cell line (homozygous deletion of the ACVR1B gene and wild- type SMAD4 gene). TGFB1 inhibited cellular growth (0.1 ng/mL, P = 0.011*; 1 ng/mL, P = 0.013*; 10 ng/mL, P = 0.0039*), but activin A did not influence the cellular growth (1 ng/mL, P = 0.65; 10 ng/mL, P = 0.93; 100 ng/mL, P = 0.82). (D) Cellular growth of Sui73 cell line (wild- type ACVR1B and SMAD4 genes). As is seen in the Sui66 cell line, both TGFB1 (0.1 ng/mL, P = 0.072; 1 ng/mL, P = 0.0087*; 10 ng/mL, P = 0.0066*) and activin A (1 ng/mL, P = 0.21; 10 ng/mL, P = 0.0018*; 100 ng/mL, P = 0.028*) inhibited cellular growth. hDEL, homozygous deletion; WT, wild-type; Columns, mean of independent triplicate experiments; Bars, SD; *P < 0.05.