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Figure 1 | Molecular Cancer

Figure 1

From: Hepatitis B virus X protein accelerates hepatocarcinogenesis with partner survivin through modulating miR-520b and HBXIP

Figure 1

HBx accelerates hepatocarcinogenesis with partner survivin. (A) The expression of survivin in the liver tissues of p21-HBx Tg mice aged 6, 12 and 18 mouths versus WT littermate mice were determined by western blotting, respectively (**P < 0.01, Student’s t test). (B) The integrations of HBx and survivin genes into the genomes of LO2 cells were validated by PCR using genomic DNA as a template. GAPDH was used as a loading control. (C) The effect of HBx and/or survivin on cell proliferation was detected by colony-formation assay (*P < 0.05, **P < 0.01, Student’s t test). (D) Tumor formation in nude mice (n = 8 per group) injected with LO2-X or LO2-X-S cells was assessed in 3 weeks. The expression of AFP was tested in the tumor tissues from mice by western blotting and IHC analysis, respectively.

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