HBx up-regulates HBXIP in HBx-Tg mice and HCC tissues with partner survivin. (A) The expression of HBXIP was detected by western blotting in LO2 and engineered cell lines (*P < 0.05, Student’s t test). (B) The expression of HBXIP in the liver tissues of p21-HBx-Tg mice aged 6, 12 and 18 mouths versus WT littermate mice were determined by western blotting, respectively (*P < 0.05, Student’s t test). (C) Expression of HBXIP was examined by IHC staining in the clinical tissues of normal liver, hepatitis, liver cirrhosis, HCC and peritumor tissues. (D, E) Correlation between relative expression of HBXIP and that of HBx (or survivin) was examined by qRT-PCR in 22 cases of HCC tissues (***P <0.001, r = 0.797 or r = 0.717; Pearson’s correlation coefficient). Data presented are from three independent experiments.