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Figure 3 | Molecular Cancer

Figure 3

From: Yin Yang-1 suppresses invasion and metastasis of pancreatic ductal adenocarcinoma by downregulating MMP10 in a MUC4/ErbB2/p38/MEF2C-dependent mechanism

Figure 3

Effects of YY1 on the proliferation, migration and invasion of BXPC-3 cells in vitro. (a) Measurement of YY1 expression in stably transfected BXPC-3 cells using qRT-PCR. BXPC-YY1 indicates YY1overexpressing BXPC-3 cells; BXPC-Vector indicates BXPC-3 cells transfected with a control vector; BXPC-YY1 shRNA indicates YY1 knockdown BXPC-3 cells; BXPC-Scramble shRNA indicates BXPC-3 cells transfected with a vector expressing Scramble shRNA. (b) Measurement of YY1 expression in stably transfected BXPC-3 cells using western blotting. The results are similar to those seen in the qRT-PCR analysis. (c) CCK-8 assays were performed. OD values from four independent experiments were assessed. (d) Cell migration and invasion assays were performed. The upper chambers were seeded with various cell lines. The membranes of the chambers were stained with 0.1% crystal violet. Scale bar, 100 μm. (e, f) Quantification of the data from Figure 3d. OD values from three independent experiments were assessed. (*represents p < 0.05, **represents p < 0.01, # represents p < 0.001, compared with the control group).

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Molecular Cancer

ISSN: 1476-4598