Figure 2

Summary of differentially expressed genes in HCT-8R cells as compared to E cells. RNA-Seq analyses were performed as described in Methods. The data are expressed as fold expression. The bar graph represents the average fold expression changes of R cells compared to E cells cultured under different conditions (PS3, Gel3, and PS17) that do not allow an E to R transition. The graphical and tabulated data summarize the up-regulated (green) as compared to down-regulated (red) genes. The composite results suggest that, most of the differentially up-regulated genes in R cells are associated with the functionalities of cell proliferation, motility, metabolism, invasive phenotype, colorectal adenocarcinoma and tumor metastasis. The differentially down-regulated genes in R cells are associated with tumor suppression and inhibition of apoptosis.