Figure 6
From: USP18 is crucial for IFN-γ-mediated inhibition of B16 melanoma tumorigenesis and antitumor immunity
Inhibition of USP18 activity in tumor cells compromises antigen-specific CTL activity. CD45.1+ C57BL/6 mice received intravenous inoculation of 1 × 106 B16-OVA-shCon and B16-OVA-shUSP18 tumor cells. At day 8, 5 × 106 activated CD45.2+ OT-1 CTLs were intravenously injected into tumor-bearing mice. On day 10, the mice were sacrificed; CD45.2+ CTLs in lung, lung draining lymph nodes, and spleen were analyzed for OT-1 tetramer expression on CD8+ T cells (gating on CD8+ cell population) (A-B), and IFN-γ and TNF-α (C), and PD-1 and KLRG1 (D).